The Tom Williams Lab at the University of Dayton
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Protocols

Williams’ Lab Recipes

Qubit 4

Annealing Oligos Protocol

Bacterial Transformation Protocol

Biotin 3′ End DNA Labeling Kit

Chemiluminescent Nucleic Acid Detection Module (Thermo Scientific, 89880)

Claxton et al., Laser scanning confocal microscopy

Thermo Scientific Miniprep Protocol

PCR “Synthesis by Overlap Extension (SOEing) Protocol

Poster Printing at Keck Environment Lab

Preparation of Z-competent cells

Purifying DNA fragments following restriction enzyme digests or PCR

Purifying Large DNA Fragments following Electrophoresis

Purification of total DNA from insects using the DNeasy® Blood & Tissue Kit

Purifying Small DNA Fragments following Electrophoresis

optimizing-restriction-endonuclease-reactions

Drosophila Sugar Food recipe

JTH gDNA Protocol (06.25.2018)

Salt-Out gDNA protocol

UVP Gel Doc Protocol

Using the Gel Documentation System to Image

Fluoview 1000

Notes on good pH meter use and storage

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